Higham, Jason (DEW)Jason.Higham@sa.gov.au​You​OFFICIALGood afternoonThanks for your inquiry to our website regarding whether our department uses eDNA technology. In looking through the available information, we do not currently use eDNA testing in South Australia routinely but some of South Australia’s Natural Resource Management Boards, the Landscape Boards have used eDNA in their projects.Please see https://www.landscape.sa.gov.au/hf/ne... and https://www.greenadelaide.sa.gov.au/n... for more information on these projects.As I understand it, these projects are not routinely screening for Tasmanian tiger/thylacine on the list of species you screen. I would recommend you visit https://www.landscape.sa.gov.au/ to seek further information from themKindest regardsJaseJason HighamManager, Conservation and Threatened Species Unit National Parks & Wildlife ServiceDepartment for Environment and WaterP (08) 8207 7736| M 0409 098 535 | Jason.higham@sa.gov.au81-95 Waymouth Street, Adelaide, SA, 5001GPO Box 1047, Adelaide, SA, 5001environment.sa.gov.au | parks.sa.gov.auSouth Australian Government Department for Environment and Water.Sadly it’s looking unlikely living Tasmanian Tigers exist in 2025 in the mainland Australian states of NSW and Victoria given vast areas have now been surveyed for Tasmanian tiger DNA twice in these states and combined with the massive loss of habitat that occurred with the 2019/2020 bush fires.https://www.envirodna.com/resources/n...