Join us for an insightful conversation with Dr. Ryland Giebelhaus a chromatographer and metabolomics researcher from the University of Victoria, as he breaks down fecal microbiota transplantation (FMT)—a revolutionary treatment changing lives by restoring healthy gut bacteria. In this episode, you’ll learn:- What FMT is and why it’s a game-changer for treating recurrent Clostridioides difficile infections and other gut-related diseases - How cutting-edge techniques like comprehensive two-dimensional gas chromatography (GC×GC-TOFMS) enable detection of key metabolites such as short-chain fatty acids, linking gut microbiome function to health - Practical challenges of analyzing biological samples, including sample prep and maintaining bacterial viability for research and clinical use - The role of advanced chromatography and mass spectrometry in monitoring FMT product stability, efficacy, and future therapeutic design - Why data science and coding skills are essential for next-generation metabolomics and microbiome research - The exciting future of multidimensional separations (GC×GC, LC×LC) in expanding our understanding of complex biological systems Whether you’re an undergraduate entering analytical chemistry or a researcher curious about the intersection of microbiology and metabolomics, this episode offers valuable perspectives on innovative science improving patient health.🔬 Don’t forget to like, subscribe, and click the bell for more expert interviews and breakthroughs in chromatography, mass spectrometry, and analytical sciences.#FMT #FecalMicrobiotaTransplant #Metabolomics #GCxGC #Chromatography #GutHealth #MicrobiomeResearch #MassSpectrometry #AnalyticalChemistry #Microbiota

Join us in this fascinating episode of Concentrating on Chromatography as Dr. Rachael Guenter, PhD, shares her eight-year journey researching neuroendocrine tumors. Discover how her background in plant pathology and engineering shaped her translational medicine approach, and learn about the crucial role of mass spectrometry in uncovering protein-level insights in these rare cancers. Dr. Guenter also discusses the challenges of translating lab discoveries into clinical trials, the promise of spatial mass spectrometry for tumor heterogeneity, and the importance of collaboration between scientists, clinicians, and patients. Whether you're interested in cancer biology, mass spec applications, or cutting-edge cancer research, this episode delivers valuable perspectives and inspiring advice from a leading expert in the field. Subscribe for in-depth scientific conversations and cutting-edge chromatography content every week. #NeuroendocrineCancer #MassSpectrometry #CancerResearch #ChromatographyPodcast #TranslationalResearch #SpatialMassSpec #DrRachaelGuenter

Join analytical chemist Dr. M. Farooq Wahab from the University of Texas at Arlington as he breaks down the science behind chromatographic peak shapes in this episode of the Concentrating on Chromatography podcast. Perfect for undergraduate chemists and researchers alike, this conversation covers:- What makes an ideal chromatographic peak shape and why it matters - Common reasons your HPLC peaks might show tailing, fronting, or distortion - Practical tips for diagnosing and troubleshooting peak shape problems - How to use visual and digital tools (like Excel) to analyze your chromatogram data beyond basic numbers - The importance of baseline correction, denoising, and signal processing in chromatography - Advice for students and early researchers aiming to excel in analytical chemistryDr. Wahab shares lively insights from his research and decades of experience, revealing the underlying physics, chemistry, and engineering behind the peaks you see—and why peaks tell the story of your chromatography.🔬 Whether new to HPLC or looking to deepen your understanding, this episode demystifies a critical topic that impacts every chromatographer.Don’t forget to like, subscribe, and ring the bell for more expert chromatography content! Subscribe here: https://www.youtube.com/@UCU9oYSK_peqfuCrwpnv4fqA HPLC peak shapes, chromatography troubleshooting, peak tailing, peak fronting, chromatographic peak analysis, analytical chemistry podcast, undergrad chromatography, baseline correction HPLC, signal processing chromatography, Farooq Wahab, Concentrating on Chromatography, chromatography tips, peak shape Gaussian, chromatography data analysis

Join us for an insightful conversation with Pernilla Sörme, Technical Program Director at My Green Lab, as we explore how one organization is transforming laboratories worldwide into leaders in environmental sustainability. Discover the driving mission behind My Green Lab, the measurable impact on research and industry, and real-world tips for saving energy and reducing plastic waste in scientific labs. Pernilla shares how behavior change, grassroots and leadership buy-in, and a community-driven approach are helping thousands of labs certify their sustainability practices—and why this matters for the future of science, the planet, and organizational success. Whether you’re in academic research or commercial R&D, this candid interview explains why sustainability, reputation, and cost savings can go hand-in-hand through My Green Lab Certification, ACT Eco-label, and more. Watch for practical advice, industry milestones, and a vision for science that benefits people and the planet.

In this episode of the Concentrating on Chromatography podcast, host David Oliva interviews Ainslie Chen about her cutting-edge research on hemoglobin variant testing using liquid chromatography-high resolution mass spectrometry (LC-HR-MS). Ainslie discusses her novel method for separating and identifying hemoglobin subunits with mass shifts less than 1 Da, overcoming critical limitations in conventional clinical assays.Hear firsthand how her team developed an LC-HR-MS workflow using a C4 reversed-phase column, tackled technical challenges (including why C18 columns fell short), and achieved unprecedented sensitivity for variant detection in both adult and neonatal samples. The conversation touches on the potential of mass spectrometry to revolutionize clinical diagnostics, the importance of reproducibility, barriers to adoption in routine labs, and how teamwork drives scientific innovation.Perfect for analytical chemists, clinical laboratorians, and anyone interested in the future of protein variant analysis, this episode also features Ainslie’s advice for early-career scientists and insights from her first ACS poster presentation.Key Highlights:- Motivation behind improving hemoglobinopathy testing - Technical advantages of LC-HR-MS over gene sequencing and CE - The crucial role of the C4 column in resolving challenging samples - Critical parameters for reproducible LC-HR-MS workflows - Barriers and outlook for clinical adoption of advanced mass spectrometry - The value of teamwork and mentorship in scientific research - Direct insights from recent ACS conference experiencesIf you’re curious about where analytical chemistry meets clinical medicine and the innovations shaping the next generation of diagnostic tools, don’t miss this illuminating interview!#LCHRMS #HemoglobinVariants #AnalyticalChemistry #ClinicalDiagnostics #Chromatography #MassSpectrometry

Join us for a fascinating conversation with Dr. Subhoja Chakraborty from the University of Central Florida, whose cutting-edge research targets the global challenge of malaria drug resistance. Dr. Chakraborty shares her journey from structure-based drug design during her PhD to her recent collaborative work repurposing human kinase inhibitors as rapid, selective antimalarials against drug-resistant Plasmodium falciparum strains.In this episode, you'll learn:- How structure-guided approaches reveal potential drug targets, including cysteine proteases like falcipain-2, and kinases implicated in malaria's complex life cycle.- The power of repurposing kinase inhibitors originally developed for cancer and other diseases, focusing on compound 12, which blocks hemozoin formation and protein kinase 6—key steps in parasite survival.- Lab techniques such as affinity, gel filtration, and size exclusion chromatography for recombinant protein purification—and how troubleshooting is crucial for high-yield, stable complexes.- Advances in mass spectrometry for characterizing protein interactions, validating drug targets, and mapping molecular pathways pivotal to malaria therapeutics.- Tips and lessons for new researchers facing the challenges of sample preparation, complex protocols, and working as part of a collaborative team.Dr. Chakraborty’s insights bridge the gap between basic science and translational drug discovery, offering hope for more effective, targeted malaria treatments in a world where resistance to frontline therapies threatens millions.Referenced Papers:- "Plasmodium falciparum protein kinase 6 and hemozoin formation are inhibited by a type II human kinase inhibitor exhibiting antimalarial activity" (Cell Chemical Biology, 2025)- "New insights of falcipain 2 structure from Plasmodium falciparum 3D7 strain" (BBRC, 2022)- "Structure-Based Optimization of Protease−Inhibitor Interactions to Enhance Specificity of Human Stefin‑A against Falcipain‑2 from the Plasmodium falciparum 3D7 Strain" (Biochemistry, 2023)If you’re a student, scientist, or simply passionate about advances in infectious disease research, this episode offers actionable insights, inspiring advice, and a front-row seat to innovation in combating malaria.Subscribe for more interviews with pioneering scientists in chemistry, biology, and biomedical research!#MalariaResearch #StructuralBiology #DrugDiscovery #KinaseInhibitors #Proteomics #Chromatography #MassSpectrometry #Falcipain2 #Antimalarial #ScientificCareers #UCFResearch

In this special episode of Concentrating on Chromatography, host David Oliva steps into the guest role to share his journey as featured on the Lab Matters podcast. David discusses his transition from a passion for business and economics to leading Organomation, a renowned manufacturer of laboratory instrumentation and sample preparation tools. Organomation's flagship products—like the N-EVAP, MULTIVAP, and MICROVAP nitrogen evaporators—help scientists accelerate the sample concentration process. These instruments utilize gentle nitrogen gas flow and precise temperature control, enabling labs to prepare sensitive samples with accuracy and efficiency. David explains how these technologies are trusted by academic, government, and commercial laboratories worldwide, including leading analytical instrument manufacturers.

Listeners will gain insight into how learning agility and transferable skills drive success across industries, and why David is devoted to making scientific workflows more sustainable and less wasteful. Explore the practical impact of Organomation’s solutions—from facilitating complex analyses to reducing environmental impact—and discover what it takes to lead innovation in sample preparation. If you care about chromatography, scientific careers, or the future of lab technology, this episode is essential listening. Find us at ConcentratingOnChromatography.com or on LinkedIn.

Join us for an exciting deep dive with Isaac Plutzer, a graduate researcher at Washington University in St. Louis, as he explores the mysterious "dark kinome"—the hundreds of under-studied protein kinases with high potential for therapeutic discovery. In this interview, Isaac explains what kinases are and why a substantial fraction remain enigmatic, despite being critical regulators of cellular processes.

Isaac shares how his lab combines innovative proximity labeling and phosphoproteomics techniques with advanced mass spectrometry to systematically map the interactors, substrates, and signaling pathways of these mysterious enzymes. The conversation covers:

• What makes a kinase “dark” and why it matters for both basic biology and drug development

• How proximity labeling and mass spectrometry workflows (including TMT-based and DIA-based approaches) are expanding our map of protein interactions

• The unique challenges of complex data analysis—including imputation of missing values—and the evolving computational landscape

• Translational opportunities for new kinase-targeting drugs, and a look at the cutting edge of single-cell proteomics and automation

• Personal reflections on scientific discovery, experimental setbacks, and advice for young scientists entering the rapidly growing fields of proteomics and systems biology

Whether you’re passionate about biochemistry, analytical technology, or excited by scientific frontiers, this interview offers approachable insights into how next-generation tools are rewriting our understanding of the cell.

Listen in and be inspired by the thrill of the unknown, the promise of mass spectrometry, and the possibilities for the future of biomedical research!