Welcome to Pathocast In Review – S1. Ep6 – The sixth instalment explore how maternal biology shapes the health and development of the next generation. We unpack the maternal immune mechanisms that support a successful pregnancy, focusing on the pivotal role of regulatory T cells (Tregs) in preventing miscarriage and ensuring tolerance in subsequent pregnancies. We then shift to the maternal microbiome, examining emerging evidence on how a mother’s microbial community can influence the neurological development of her newborn. Intriguingly, we discuss research showing that microbiomes from older mothers may impart subtle developmental impairments.

Papers in Review:

1. Pregnancy-acquired memory CD4+ regulatory T cells improvepregnancy outcome in mice (DOI: 10.1038/s41467-025-61572-w).

2. Maternal dysbiosis produces long-lasting behaviouralchanges in offspring (DOI: 10.1038/s41380-024-02794-0)

Moderator: NelisaMakaleni (@nelly_saciar)

Panelists: Blessing Moses (@emeraldeey) and ValenciaChauke

Production: Zd Creations

Creator: Immun-Vir JC

Music: iLethu (@Mphephethwa_sa)

Welcome to Pathocast In Review – S1. Ep5 - On this fifth instalment we explore new evidence showing how HIV infection accelerates biological ageing, not just in years lived, but deep in the molecular architecture of antibodies revealing that changes in antibody glycosylation can predict ageing long before clinical signs appear, offering a molecular window into how chronic inflammation reshapes immune function. We then turn to obesity, a condition now understood to reprogram adipose tissue in lasting ways. Even after weight loss, fat tissue retains a form of metabolic memory, driving inflammation and accelerated cellular senescence. But here’s the hopeful part, this ageing process can be partially reversed by weight loss.Papers in Review:

1. Immunoglobulin G N-glycan markers of accelerated biological aging during chronic HIV infection. (DOI: 10.1038/s41467-024-47279-4).

2. Selective remodelling of the adipose niche in obesity and weight loss. (DOI: 10.1038/s41586-025-09233-2).

Moderator: Adijat Jimoh (@deejah_deej)

Panelists: Wealth Okete (@de_dew001) and Sonwabile Dzanibe (@⁠⁠⁠⁠⁠⁠⁠⁠sonwa_dzanibe⁠⁠)

Production: Zd Creations

Creator: Immun-Vir JC

Music: iLethu (@Mphephethwa_sa)

Welcome to Pathocast In Review – S1. Ep4 - From AgeingImmunity to Infant Gut Resistome. On this fourth instalment, we unpack how ageing reshapes the immune landscape, where multi-omic profiling using single cell RNA-sequencing and mass cytometry reveals that CD8 T cells bear the brunt of age-related changes, while children show a surprising enrichment of cytotoxic B cells. Then, we turn to a longitudinal mother-infant analysis exploring factors that contribute to the development of infant gut resistome, yes the load of antibiotic-resistant genes, with Bifidobacterium being the key player in shaping the infant resistome, protecting against the accumulation of antibiotic resistance genes (ARGs) and how early cessation of breastfeeding tips the balance in the wrong direction.

Papers in Review:

1. Integrating single-cell RNA and T cell/B cell receptorsequencing with mass cytometry reveals dynamic trajectories of human peripheralimmune cells from birth to old age. (DOI: 10.1038/s41590-024-02059-6).

2. Breastfeeding and early Bifidobacterium-driven microbialcolonization shape the infant gut resistome. (DOI: ⁠⁠⁠⁠⁠⁠⁠⁠⁠10.1038/s41467-025-61154-w⁠).

Moderator: Rachael Gachogo

Panelists: Zwile Zungu (@zwilezungu) and Gadisi Nthambeleni(@⁠⁠⁠⁠ gadisinth)

Production: Zd Creations

Creator: Immun-Vir JC

Music: iLethu (@Mphephethwa_sa)

Welcome to Pathocast In Review – S1. Ep3 - Immune Harmony and Disruption: IgA in the Gut, and Gestational Diabetes Risks GBS. The third episode explores how secretory IgA shapes intestinal immune balance by limiting viral colonisation, and how its absence triggers a compensatory expansion of CD8 T cells. We also examine how gestational diabetes disrupts maternal immunity and alters the vaginal microbiota, amplifying the harmful effects of Group B Streptococcus colonisation during pregnancy.

Papers in Review:1. Immunoglobulin A controls intestinal virus colonization to preserve immune homeostasis (DOI: ⁠⁠10.1016/j.chom.2025.03.004).

2. Gestational diabetes augments group B Streptococcus infection by disrupting maternal immunity and the vaginal microbiota (DOI: ⁠⁠⁠⁠10.1038/s41467-024-45336-6⁠).

Moderator: Wealth Okete (@wealthokete_)

Panelists: Rachael Gachogo and Nelisa Makeleni (@nelly_saciar⁠)

Production: Zd Creations

Creator: Immun-Vir JC

Music: iLethu (@Mphephethwa_sa)

Welcome to Pathocast In Review – S1. Ep2 - Silent Shifts: How Early Life Antibiotics and Hidden HIV Cells Shape Immunity. In this second episode, we explore the far-reaching consequences of early-life antibiotic use. Beyond driving the emergence of antibiotic resistance, these interventions disrupt the developing microbiota—blunting vaccine responses and leaving infants more vulnerable to infections. We also dive into cutting-edge research using a mouse model system to track latently HIV-infected cells. Through the HIV-1-Induced Lineage Tracing (HILT) system, we examine whether these persistent cells express distinguishing markers that could make them identifiable—and ultimately, targetable—for eradication.Papers in Review:

1. Bifidobacteria support optimal infant vaccine responses (DOI: ⁠10.1038/s41586-025-08796-4).

2. Tracking HIV persistence across T cell lineages during early ART-treated HIV-1 infection using a reservoire-marking humanized mouse model (DOI: 10.1038/s41467-025-57368-7).

Moderator: Sonwa Dzanibe (@sonwa_dzanibe)Panelists: Anna Happel and Cheval Sookram (@chevalsookram)

Production: Zd Creations

Creator: Immun-Vir JC

Music: iLethu (@Mphephethwa_sa)

Welcome to Pathocast In Review – S1. Ep1: Targeting precursor B Cells for bnAbs | Antibiotics to protect uncircumsed from HIV.
In our premiere episode, we dive into the cutting edge of HIV science. Join us as we explore the latest findings on the quest for an effective HIV vaccine—specifically, how broadly neutralising antibodies are induced by vaccines by targeting extremely rare precursor B cells.

We also unpack new research into how antibiotics might offer an unexpected tool in HIV prevention for men who opt not to undergo circumcision. It's a nuanced discussion at the intersection of immunology, microbiota, and global health strategy.

Papers in Review:

1. Diverse priming outcomes under conditions of very rare precursor B cells (DOI: 10.1016/j.immuni.2025.03.003).

2. Impact of antimicrobials on penile HIV susceptibility and immunology in uncircumcised men: A randomized phase 1/2 clinical trial (DOI: 10.1016/j.xcrm.2024.101705).

Moderator: Sherazaan Ismail

Panelists: Sonwa Dzanibe (@sonwa_dzanibe) and Adijat Jimoh (@deejah_deej)

Production: Zd Creations

Creator: Immun-Vir JC

Music: iLethu (@Mphephethwa_sa)