Welcome to Pathocast In Review – S1. Ep4 - From AgeingImmunity to Infant Gut Resistome. On this fourth instalment, we unpack how ageing reshapes the immune landscape, where multi-omic profiling using single cell RNA-sequencing and mass cytometry reveals that CD8 T cells bear the brunt of age-related changes, while children show a surprising enrichment of cytotoxic B cells. Then, we turn to a longitudinal mother-infant analysis exploring factors that contribute to the development of infant gut resistome, yes the load of antibiotic-resistant genes, with Bifidobacterium being the key player in shaping the infant resistome, protecting against the accumulation of antibiotic resistance genes (ARGs) and how early cessation of breastfeeding tips the balance in the wrong direction.

Papers in Review:

1. Integrating single-cell RNA and T cell/B cell receptorsequencing with mass cytometry reveals dynamic trajectories of human peripheralimmune cells from birth to old age. (DOI: 10.1038/s41590-024-02059-6).

2. Breastfeeding and early Bifidobacterium-driven microbialcolonization shape the infant gut resistome. (DOI: ⁠⁠⁠⁠⁠⁠⁠⁠⁠10.1038/s41467-025-61154-w⁠).

Moderator: Rachael Gachogo

Panelists: Zwile Zungu (@zwilezungu) and Gadisi Nthambeleni(@⁠⁠⁠⁠ gadisinth)

Production: Zd Creations

Creator: Immun-Vir JC

Music: iLethu (@Mphephethwa_sa)