#1 — Hepatocytes are liver cells that are essential for its function. They are also essential for certain branches of drug testing and diagnostics.

However, they have irregular shapes, multiple nuclei, and can clump, which makes them difficult to process and count accurately.

In this episode, we explain how to prepare the best hepatocyte samples possible.

We also explain the latest automated machine learning-based tools that make hepatocyte counting easy and do essential sample-based calculations for you, removing tedious math. See the full article here. [1]

For more details on the machine learning technology discussed in this episode, read this technical note. [2] And to hear more from DeNovix experts on hepatocyte counting, watch their webinar Automated Hepatocyte Counting: New Technology for Simple, Reliable Counts. [3]

Resources:
1. Hepatocyte Counting Methods: From Manual Counts to Fast, Accurate Automation. Available at: https://bitesizebio.com/83141/hepatocyte-counting-methods/
2. TN 228 Automated Counting of Hepatocytes. Available at: https://www.denovix.com/tn-228-automated-counting-of-hepatocytes/?utm_source=bitesizebio&utm_medium=technicalcontent&utm_campaign=hepatocytes
3. Automated Hepatocyte Counting: New Technology for Simple, Reliable Counts. Available at: https://events.bitesizebio.com/automated-hepatocyte-counting-new/room

#114 — High-quality nuclei extraction is pivotal for many genetic and epigenetic studies, including single cell RNA-seq to describe transcriptomic profiles and gene expression dynamics and ATAC-seq to determine chromatin accessibility.

But nuclei samples are extremely delicate and can be challenging to prepare and count.

In this episode, we provide some practical tips to prepare high-quality nuclei extracts and tailor your extraction protocol to your sample.

We also compare methods to count nuclei, assess their quality, and demonstrate how automated nuclei counting can improve your workflow.

Check out the original article for a graphic that shows you the level of purity and quality you should aim for with nuclei extraction. [1] If you want to learn more about automated nuclei counting, download this free eBook. [2] Read this article to learn how to count cells quickly using fluorescence microscopy. [3] And to hear directly from DeNovix experts on nuclei counting, watch their webinar Optimizing Nuclei Extraction & Counting for Single Cell Sequencing. [4]

Resources:
1. Single Cell Sequencing: Tips to Optimize Nuclei Extraction and Counting. Available at: https://bitesizebio.com/78877/optimizing-nuclei-extraction/
2. Automated Counting of Isolated Nuclei. Available at: https://www.denovix.com/ebook/automated-counting-of-isolated-nuclei?utm_source=bitesizebio&utm_medium=technicalcontent
3. Quick and Easy Automatic Cell Counting in 4 Steps. Available at: https://bitesizebio.com/30184/quick-easy-automatic-cell-counting/
4. Optimizing Nuclei Extraction & Counting for Single Cell Sequencing. Available at: https://events.bitesizebio.com/optimizing-nuclei-extraction/room

#113 — Learning how to respond to criticism is an unavoidable aspect of grant applications. Follow all instructions from funding agencies to avoid immediate rejection.

This episode of Mentors At Your Benchside gives you some simple strategies to ensure you respond to criticism in a constructive and proportionate way.

For example, identifying reviewers can help you tailor your responses, but don't mention them by name! Plus, using a two-column document to systematize the criticisms and responses is a great way to organize feedback and distance yourself emotionally from it.

Hit play for more tips, check out the original article for links to helpful resources [1], and check out our complete guide to funding series for advice on getting your research funded—directly from a former NIH grant reviewer [2].

Resources:
1. Personal Advice on How to Respond to Criticism of Your Grant Proposal Reviews. Available at: https://bitesizebio.com/80095/respond-to-criticism/
2. The Complete Guide to Funding I: How to Become an Expert at Getting Funded. Available at: https://events.bitesizebio.com/the-complete-guide-to-funding-i-how/join