Precision oncology research increasingly depends on finding the right biomarker and choosing the right tool to measure it.

In this episode of Absolute Gene-ius, Dr. Javier Hernández Losa, leader of the Molecular Biology Laboratory at Vall d’Hebron University Hospital, joins Lisa Crawford and Jordan Ruggieri to discuss the evolving world of biomarker research. Javier explores the growing range of protein, DNA, RNA, and other molecular biomarkers, along with challenges posed by FFPE tissue, limited sample material, and liquid biopsy. He also explains how technology selection depends on the biological question: qPCR and digital PCR can be valuable for highly specific, sensitive analysis of a small number of known targets, while NGS can support broader profiling and discovery. The conversation also covers molecular residual disease, orthogonal validation, assay design, methylation, and the potential of combining multiple layers of molecular information.

In Career Corner, Javier reflects on 25 years in oncology research, the mentor who helped launch his career, and why young scientists should stay open to new technologies, questions, and directions. He also shares the familiar graduate-school promise that results will always be ready “on Monday.”

Visit the Absolute Gene-ius page to learn more about the guests, hosts, and science featured in the series. Explore how Applied Biosystems™ QuantStudio™ Digital and Real-Time PCR Systems support research across neuroscience, oncology, agriculture, and more.