Python problems – digital PCR takes on the Everglades
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Python problems – digital PCR takes on the Everglades
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This episode of Absolute Gene-ius slithers into the surprising science of invasive species monitoring with Dr. Brian Bahder. A childhood love of bugs led Brian to a dynamic career in entomology and plant pathology—and eventually to tracking large reptiles in the swamps of Florida.
We dive deep into Brian’s work developing multiplex digital PCR assays to detect DNA from snakes, caimans, and other invasive species using environmental samples like soil and water. He explains how this technology enables detection even after the animals are gone, and how sampling strategy, environmental variables, and experimental design are critical to getting reliable data. He also compares qPCR and digital PCR, emphasizing how each has its place depending on sensitivity, speed, and sample complexity.
In the career corner, Brian shares how his academic journey was shaped by travel, risk-taking, and a healthy dose of failure. From surfing and skateboarding to discovering new species and running a diagnostic clinic, his path reminds us that science thrives on curiosity—and that even mistakenly detecting your own DNA can teach you something.
Visit the Absolute Gene-ius pageto learn more about the guests, the hosts, and the Applied Biosystems QuantStudio Absolute Q Digital PCR System.